A revamped front row, a new flyhalf, and a new debutant headline the Wallaroos side that is set to make an early World Cup statement against the USA.
Wallaroos coach Jo Yapp has made four changes to the starting side that went down to the Black Ferns in Newcastle, with two new weapons coming onto the bench.
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Bree-Anna Browne comes back into the side after missing out in Newcastle, being joined by Tania Naden after her strong showing in the second half to partner Eva Karpani.
Katalina Amosa will be rotated back to the bench with Bridie O'Gorman and breakout star Faliki Pohiva.
Lydia Kavoa has been ruled out for the rest of the series with a calf injury.
The locks will remain unchanged, with Yapp opting to swap Emily Chancellor in for Ashley Marsters, with the former serving as impact off the bench.
The final bench change will see Ruby Anderson in line to make her debut. Should she take the field, she will become Wallaroo #224.
The backline sees two changes, with Sevens star Tia Hinds also rested following her encouraging performance at flyhalf against the Black Ferns in Newcastle.
Yapp has opted for the more experienced option at ten, with Faitala Moleka shifting back in from fullback. Caitlyn Halse drops back into fullback after recovering from a hamstring injury.
All other positions remain unchanged, with Desiree Miller and Charlotte Caslick completing the back three, Cecilia Smith and Georgina Friedrichs will remain in the centres, with unchanged support from the bench.
"There were some positive signs last week for us to build on and also some areas we have needed to hone in and really focus on," Yapp said in the official press statement.
"USA will provide us with another challenge on Saturday and given we play them at World Cup it will be a good test to see where we’re placed.
"Caitlyn Halse returns from injury this weekend and we’re also excited for Ruby Anderson to make her debut.
"The Wallaroos haven’t played in Canberra since 2008 so the players are looking forward to playing at GIO Stadium for the first time."
Reds winger Caitlin Urwin has also been called into camp, with ACT Brumbies winger Biola Dawa and NSW Waratahs hooker Adiana Talakai also part of the extended squad.
1. Bree-Anna Browne (#193 – Brothers Rugby) – 10 caps
2. Tania Naden (#197 - Uni-North Owls) – 21 caps
3. Asoiva (Eva) Karpani (#171 - Southern Suburbs) – 33 caps
4. Kaitlan Leaney (#179 - CSU Marlins) – 26 caps
5. Michaela Leonard (#168 - Tuggeranong Vikings) – 34 caps
6. Siokapesi Palu (c) (#194 - Rockdale Rangers) – 18 caps
7. Ashley Marsters (#117 - Boroondara) – 36 caps
8. Tabua Tuinakauvadra (#200 - Orange Emus) – 13 caps
9. Layne Morgan (#188 - Merewether Carlton) – 31 caps
10. Faitala Moleka (#199 - Blacktown Scorpions) – 18 caps
11. Desiree Miller (#204 - Eastern Suburbs) – 14 caps
12. Cecilia Smith (#190 - Leeton Dianas) – 21 caps
13. Georgina Friedrichs (#178 - Wests Bulldogs) – 30 caps
14. Charlotte Caslick (#220 - Wests Bulldogs) – 2 caps
15. Caitlyn Halse (#210 - Camden Rams) – 7 caps
Reserves
16. Katalina Amosa (#218 - Southern Districts) – 2 caps
17. Faliki Pohiva (#219 - Blacktown Scorpions) – 1 cap
18. Bridie O’Gorman (#180 - Sydney University) – 28 caps
19. Emily Chancellor (#158 - Sydney University) – 25 caps
20. Ruby Anderson (Avoca Beach Rugby) – debut
21. Natalie Wright (#213 - Wests Bulldogs) – 5 caps
22. Trilleen Pomare (#155 - Wanneroo) – 35 caps
23. Lori Cramer (#172 - University of Queensland) – 28 caps
Players unavailable for selection
- Piper Duck (shoulder)
- Brianna Hoy (knee)
- Lydia Kavoa (calf)
- Atasi Lafai (knee)
- Leilani Nathan (knee)
- Maya Stewart (knee)
- Bienne Terita (hamstring)
- Samantha Wood (back)